Platelet function analyses. (A) Platelet aggregation/agglutination was stimulated with collagen (2.0 µg/mL), ristocetin (1.2 mg/mL), adenosine 5'-diphosphate (4.0 µmol/L), and epinephrine (8.0 µmol/L). Analysis was performed with an aggregometer (APACT; Labor Fibrintimer). (B-C) Flow cytometric quantification of platelet granule secretion was stimulated using increasing concentrations of thrombin (0, 0.05, 0.1, 0.2, 0.5, and 1.0 U/mL). After fixation cells were washed and incubated with fluorescein isothiocyanate-conjugated anti-CD62 (B) or fluorescein isothiocyanate-conjugated anti-CD63 (C). Surface fluorescence was analyzed with a flow cytometer (FACS Calibur; Becton Dickinson). Analyses were performed with patient’s platelets and platelets from a healthy control.