Homeostatic T-cell proliferation does not require FA metabolism. (A) BoDipy uptake was measured in B6 Ly5.2 donor T cells from either allogeneic (GVHD) or syngeneic (Syn) recipients as in Figure 1A. n = 3 mice/group and represent 3 independent experiments. Plots are gated on CD45.1⁺, TCR-β⁺ cells. (B) Donor T cells from allogeneic (GVHD) or syngeneic recipients were purified by flow-sorting 7 days after BMT and cell lysates probed via western blotting for PGC-1α as in Figure 2. (C) Syngeneic recipients were given a single intraperitoneal dose of PBS (white) or etomoxir (gray) 7 days post-BMT and the number of donor T cells measured 16 hours later (compare with Figure 6B). n = 8 mice/group, 1 of 2 independent experiments shown. **P < .005, n.s., not significant.