American Society of Hematology

Figure 4

Allogeneic T cells from a minor histocompatibility model increase FA transport and up-regulate CPT2 and PGC1-α (A) 3 × 106 B6 Ly5.2 (CD45.1+) T cells were transplanted into irradiated C3H.SW as detailed in “Methods.” BoDipyC1-C12 uptake was measured as in Figure 1, with allogeneic T cells shown in solid black line and naïve in shaded gray. The percentage of BoDipyHi cells and the MFI of BoDipy was further quantitated (n = 9 GVHD, n = 3 naïve) and represent 2 independent experiments. (B) 105 T cells from naïve donors or minor histocompatibility allogeneic recipients (GVHD) were purified by flow-sorting and cell lysates probed for CPT2 by western blotting as in Figure 2. (C) PGC-1α levels in naïve donor or day 7 allogeneic T cells. Lamin B1 served as a loading control. n = 3 mice per lane. **P < .004, ***P < .0005.

Allogeneic T cells from a minor histocompatibility model increase FA transport and up-regulate CPT2 and PGC1-α (A) 3 × 10⁶ B6 Ly5.2 (CD45.1⁺) T cells were transplanted into irradiated C3H.SW as detailed in “Methods.” BoDipy_C1-C12 uptake was measured as in Figure 1, with allogeneic T cells shown in solid black line and naïve in shaded gray. The percentage of BoDipy^Hi cells and the MFI of BoDipy was further quantitated (n = 9 GVHD, n = 3 naïve) and represent 2 independent experiments. (B) 10⁵ T cells from naïve donors or minor histocompatibility allogeneic recipients (GVHD) were purified by flow-sorting and cell lysates probed for CPT2 by western blotting as in Figure 2. (C) PGC-1α levels in naïve donor or day 7 allogeneic T cells. Lamin B1 served as a loading control. n = 3 mice per lane. **P < .004, ***P < .0005.

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