Platelet MP-derived miR-223 can regulate HUVEC gene expression at the protein level. (A) Protein extracts prepared from HUVEC, incubated or not with MPs derived from thrombin-activated platelets for up to 48 or 96 hours, were analyzed by immunoblotting (IB) using anti-F-box and WD-40 domain protein 7 (anti-FBXW7) (left upper panel; n = 3 experiments), anti-EFNA1 (right upper panel; n = 1 experiment) or anti-actin (lower panels) antibody, which was used as a loading control. The data were analyzed by densitometry and expressed as a percentage of control. (B) Proposed model for the intercellular transfer of functional Ago2•microRNA complexes between activated platelets and endothelial cells through the release of MPs. RNA pol II, RNA polymerase II.