Splenic CD8⁺T-cell phenotype. Splenic CD8⁺ T-cell phenotype was characterized for the expression of (A) HLA-DR and the cytotoxic proteins granzyme B and perforin. (B) The expression of the chemokine receptor CCR7, the adhesion molecule CD62L, and the memory markers CD27 and CD28 were also assessed. Data are summarized in dot plots representing the expression of each marker among CD8⁺ T cells, in 9 controls (black circles), 11 RTX-untreated patients (black squares), and 12 RTX nonresponders (black triangles). The horizontal bar represents the median with the interquartile range. P values were derived by Mann-Whitney U test. *P < .05; **P < .01; ***P < .001. NS, nonsignificant.