Sensitization of primary Eµ-Myc cells to ABT-737–induced death by CR mimetics is independent of Puma or Noxa. (A) MEF and indicated Eµ-Myc cells were incubated with varied doses of ABT-737 for 20 hours. Dead cells were counted by PI staining and analyzed by FACS. The insert shows Mcl-1 expression. (B) Eµ-Myc/Noxa^−/− and Eµ-Myc/Puma^−/− cells were incubated with 2DG 100 µg/mL or LND 100 µM for 20 hours, and the levels of Mcl-1 were analyzed by immunoblot. HSP60 was used as a loading control. Quantifications of Mcl-1 over HSP60 levels are indicated. (C-D) Eµ-Myc/Puma^−/− and Eµ-Myc/Noxa^−/− cells were treated with the indicated doses of ABT-737 with or without 2DG 100 µg/mL (C) or LND 100 µM (D) for 20 hours. Cell death was determined as in Figure 3A. The results represent the mean ± SD from 3 independent experiments. *P < .05, **P < .01, ***P < .001.