AND TCR transgene fails to rescue developmental arrest of DN-IkTg CD4SP thymocytes. (A) Thymocyte profiles of AND and AND.DN-IkTg mice. Total thymocytes were assessed for CD4/CD8 expression, and TCR Vα11 expression was determined on gated CD4SP cells. HSA and TCRβ expression was assessed on Vα11^hi CD4SP thymoycytes. Data are representative of at least 7 independent experiments with 7 AND and 9 AND.DN-IkTg mice. (B) Intracellular Ki-67 staining in HSA^hi and HSA^lo TCRβ⁺ CD4SP thymocytes of AND and AND.DN-IkTg mice. Data are representative of 3 independent experiments. (C) Total thymocyte numbers from AND and AND.DN-IkTg mice. The graph shows the mean ± SEM from 7 independent experiments with 7 AND and 9 AND.DN-IkTg mice.