Immunocytochemical colocalization of fibrin(ogen) with sphingomyelin-rich rafts in central region of adhered platelets in the presence of thrombin. (A) (a) mCherry-lysenin-positive sphingomyelin (SM)-rich rafts (red), (b) fPEG-Chol-positive cholesterol-rich rafts (green), (c) phase-contrast cell morphology, (d) merged images of (a-c). (B) colocalization of fibrin (red) with green fluorescent protein–lysenin-positive SM-rich rafts (green). Scale bar, 5 μm. (C) Photoactivated localization microscopy and direct stochastic optical reconstruction microscopy (PALM/dSTORM) imaging of lysenin-positive SM-rich rafts (green) and fibrin(ogen) (red). The white line represents the shape of an adhered human platelet.