miR-30c is a direct target of C/EBPα. (A) Schematic representation of examined putative C/EBPα binding sites (α) in the promoter regions of miR-30c-1, the host gene NFYC, and miR-30c-2 (primer #1-#4). (B) Chromatin derived from K562-C/EBPα-p42-ER cells induced for 4 hours with β-estradiol was immunoprecipitated with anti-C/EBPα or IgG antibodies. Recovered DNA was PCR amplified with different primers specific for C/EBPα-binding amplificon (primers #1-#4). (C) Q-RT-PCR with primers for indicated regions with the samples from (B). Shown is the fold change in binding affinity of the anti-C/EBPα antibody normalized to IgG from β-estradiol (active C/EBPα) –treated vs control vehicle–treated cells. Total data are represented as mean ± SD from 2 independent experiments.