Figure 3
Figure 3. Regulation of miR-30c in inducible C/EBPα-knockout mice. (A) Q-RT-PCR for miR-30c in sorted mouse bone marrow cell populations. Values were normalized with snoRNA-135. Total data are represented as mean ± SD from 3 independent mice. *P ≤ .05. LSK (Lin– cKit+ Sca1− contains hematopoietic cells, both LTHSCs and STHSCs), CMP (common myeloid progenitors), GMP (granulocyte-macrophage precursors), MEP (megakaryocyte-erythrocyte precursors), and granulocytes. (B-C) Q-RT-PCR for C/EBPα (B) and miR-30c (C) was carried out using bone marrow cells derived from poly(I:C)-injected C/EBPαfl/fl;Mx-Cre (C/EBPαΔ/Δ) and C/EBPαfl/fl control. Values were normalized with GAPDH and snoRNA-202, respectively, and further to the expression of C/EBPαfl/fl control. Total data are represented as mean ± SD from 5 independent mice. **P ≤ .005.

Regulation of miR-30c in inducible C/EBPα-knockout mice. (A) Q-RT-PCR for miR-30c in sorted mouse bone marrow cell populations. Values were normalized with snoRNA-135. Total data are represented as mean ± SD from 3 independent mice. *P ≤ .05. LSK (Lin cKit+ Sca1 contains hematopoietic cells, both LTHSCs and STHSCs), CMP (common myeloid progenitors), GMP (granulocyte-macrophage precursors), MEP (megakaryocyte-erythrocyte precursors), and granulocytes. (B-C) Q-RT-PCR for C/EBPα (B) and miR-30c (C) was carried out using bone marrow cells derived from poly(I:C)-injected C/EBPαfl/fl;Mx-Cre (C/EBPαΔ/Δ) and C/EBPαfl/fl control. Values were normalized with GAPDH and snoRNA-202, respectively, and further to the expression of C/EBPαfl/fl control. Total data are represented as mean ± SD from 5 independent mice. **P ≤ .005.

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