LCP1 protein was identified in the nucleus, cytoplasm, and exosomes of both CLL cells and healthy B cells. (A) Immunoblot analysis of whole cell, nuclear, and cytoplasmic extracts demonstrates that LCP1 protein is present in both subcellular compartments in healthy donor and CLL B cells. Brg1 protein is used as a nuclear loading control, and β-actin is used as a cytoplasmic loading control. Quantitative analysis of band density relative to Actin or Brg1 controls is provided. (B) Confocal immunofluorescent analysis of LCP1 localization in CLL cells and healthy donor B cells confirms evidence that LCP1 is heavily represented in both the nuclear (N) and cytoplasmic (arrow) compartments of malignant and healthy B lymphocytes. (C) Purified exosomes isolated from ex-vivo cultured CLL cells or healthy B cells with or without CpG stimulation were probed for LCP1 by immunoblot. CD63 and HSP70 were used as exosome-specific loading controls.