H6PDH regulates the differentiation and immunostimulatory potential of BM-derived DCs. (A) H6pdh−/− (open histograms) or H6pdh+/+ littermate (solid gray histograms) BM-derived DCs were exposed to 2 µg/mL LPS in the presence or absence of 10−7 M A or 10−7 M B. Representative staining for MHC class II and CD86 in gated CD11c+ BM DCs from mice. (B-C) Immature DCs (iDCs) or LPS-matured BM DCs (mDCs) from H6pdh−/− or H6pdh+/+ littermate control mice were untreated or pulsed with 1 µM SIINFEKL peptide before intraperitoneal injection into B6 mice. At 7 days, the host anti-OVA response was evaluated by Kb:SIINFEKL pentamer staining and by ELISPOT assay for specific IFN-γ generation. (B) Representative dot plots of pentamer staining. (C) Summary graphs for Kb:SIINFEKL pentamer and ELISPOT evaluations. Data are pooled from 3 independent experiments.
