ESL-1 dominates E-selectin binding in hematopoietic progenitors. (A) Number of progenitors in the blood of mice transplanted with WT or ESL-1^−/− BM cells, measured as CFU-C. (B) Representative dot plot at left shows the gates used for myeloid progenitors and LSK cells among Lineage^NEG BM cells. Panels at right show representative histograms of E-selectin binding to experimental (gray) and WT-DsRed (red) BM-derived neutrophils (top panel), myeloid progenitors (middle), and LSK cells (bottom). Dashed lines show levels of binding in the presence of EDTA. (C) Quantification of E-selectin-binding to BM neutrophils, myeloid progenitors, and LSK cells. Values represent fluorescence intensity ratios relative to internal WT-DsRed competitor cells. There were 4 to 6 mice per group from 3 independent experiments. (D) Quantification of P-selectin binding in LSK cells from all groups represented as in (C). There were 4 to 5 mice per group from 3 independent experiments. Bars represent mean ± SEM. Data were analyzed by 1-way ANOVA using the Tukey multiple comparison test.