Effect of ovx on HSPC expansion in T cell–replete and T cell–deficient mice. (A-C) Effects of ovx on the relative frequency of LSK cells in WT mice, TCRβKO mice, and TCRβKO mice previously subjected to adoptive transfer of T cells. Lin⁻ cells were gated and analyzed for Sca-1 and c-Kit expression using isotype control settings. (Left) Representative flow cytometric dot plots from 1 mouse per group. The black box delineates c-Kit⁺ Sca-1⁺ cells. Parent population is Lin⁻. Data are expressed as percentage of total Lin⁻ cells. (Right) Mean ± standard error of the mean (SEM) for each group. Data are expressed as percentage of total BM mononucleated cells (BMMCs). (D) Effect of ovx on the number of BM B cells, monocytes, erythroid cells, and granulocytes. n = 10 mice per group. *P < .05 and **P < .01 compared with the corresponding sham-operated group.