CD123-CAR–redirected T cells derived from AML patients specifically lyse autologous blasts in vitro. (A) T cells from 3 AML patients were lentivirally transduced to express either CD19R, 26292, or 32716 CARs. Shown are T cell lines from AML 722 19 days posttransduction. (B) CD123 expression on target cells used in ⁵¹Cr release assay. The percentage of CD123⁺ cells and the RFI of each sample is indicated. (C) Results of 4-hour autologous killing assays using T cells engineered from 3 AML patient samples as effectors and ⁵¹Cr-labeled autologous CD34-enriched blasts as target cells. Data represent mean values of triplicate wells ± SD.