Neutrophils are required for anti-gp75 mAb therapy of melanoma. (A-F) Indicated mice were injected subcutaneously with 5 × 104 B16-luc2 cells at day 0, intravenously with 200 μg of mAb TA99 or isotype Ctrl on days 0, 1, and 2, and intraperitoneally with d-luciferin immediately before total photon flux acquisition (photons per second). Indicated mice were also injected on days −1, 1, 3, 5, and 7 with (C) 200 μg/mouse clodronate-containing liposomes (Clodronate) or (E) 300 μg/mouse anti-Gr1 mAbs, or (D) on days 0, 1, and 2 with 2 × 106 WT B.M. cells (▲). (A-F) Data are represented as mean ± SEM (n.s.: P > .05; *P < .05; **P < .01; ***P < .001) and are representative of at least 2 independent experiments (n ≥ 4). B.M., bone marrow; Ctrl, control; KO, knockout; n.s., not significant; WT, wild type.
