Expression levels of gcsfr, gcsfa, and gcsfb in embryonic and adult zebrafish. (A) qRT-PCR analysis of gcsfr in pooled whole zebrafish embryos at developmental stages listed along x-axis. (B) qRT-PCR analysis of gcsf ligands (gcsfa, black bars; gcsfb, gray bars) in pooled whole zebrafish embryos at developmental stages listed along x-axis. (C) qRT-PCR analysis of gcsfr in adult zebrafish tissues listed along x-axis. Lymphoid, myeloid, and precursor cells are different cell populations isolated by FACS from the adult WKM. (D) qRT-PCR analysis of gcsfa (black bars) and gcsfb (gray bars) in adult zebrafish tissues listed along x-axis. Lymphoid, myeloid, and precursor cells are different cell populations isolated by FACS from the adult WKM. Expression levels are relative to the housekeeping gene ef1α. All embryonic samples are biological triplicate preparations of at least 10 embryos per time point. All adult tissue samples are biological triplicate preparations from individual adult fish. All bars represent the mean of the samples, and error bars represent standard deviation.