Statins modulate PECAM-1 signaling. Platelet cell lysates were immunoprecipitated for PECAM-1 or SHP-2 and immunoblotted using anti–SHP-2 and anti-p85 antibodies, respectively. Blots are representative of 3 separate experiments and quantification normalized to loading control. Numerical data represent the percentage of change of PECAM-1/SHP-2 and SHP-2/p85 association (A-B). Phosphatidylinositol 3-kinase signaling was measured through assessment of Akt phosphorylation. Platelets stimulate with CRP-XL (1 μg mL−1) in the presence of increasing concentrations of simvastatin (1-20 μM) were analyzed by immunoblotting with an Akt/PKB phospho-specific antibody (Ser473). Equivalent protein loading was verified by reprobing for total Akt (C), t test, *P ≤ .05.
