Nfix is expressed by HSPCs. (A) Heat map displaying relative transcript levels of Nfi gene family members in HSPCs throughout mouse ontogeny, as reported by the searchable online resource found at http://daleystem.hms.harvard.edu/. E, embryonic time-point; AGM, aorta-gonads-mesonephros.. (B) Lineage⁻Sca-1⁺c-Kit⁺ , Lineage⁻Sca-1⁻c-Kit⁺, Lineage⁻Sca-1⁻c-Kit⁻, and Lineage⁺ cells were isolated from WBM by FACS and analyzed via qRT-PCR for Nfix expression relative to Tbp expression. Data represent mean ± standard deviation from 2 independent experiments.