PHD inhibitors decrease HSPC proliferation in vivo. 129Sv mice were administered DMOG (black columns) or saline (white columns) daily for 18 days. BrdU was administered in the drinking water for the last 3 days of the experiment. (A) Percentage of BrdU⁺ cells. Data are shown as means ± SD from 5 mice per group. (B) Typical BrdU incorporation flow cytometry profiles for saline-treated (gray) and DMOG-treated (black) mice in LKS⁺48⁻ HSCs, LKS⁺48⁺ and LKS⁻ HPCs. (C) Cell-cycle analysis on LKS⁺48⁺ HPCs and LKS⁺48⁻ HSCs from mice treated with DMOG or saline. (D) Representative dot plots showing the proportion of cells in each phase of the cell cycle after in vivo treatment with saline or DMOG for 18 days. Data in histograms are means ± SD from 5 different mice per group. Significance levels were calculated using a t test.