Correlation of iNKT cells with Ab production against Gal and NeuGc epitopes. B6 CD1d^−/−GalT^−/− and CD1d^−/−CMAH^−/− mice were immunized with Gal- and NeuGc-bearing rat thymocytes, and the levels of anti-Gal and anti-NeuGc Abs were then determined in their respective sera using FCM. (A-B) CD1d^−/−GalT^−/− mice showed increased anti-Gal Ab titer (both IgM and IgG subclasses) similar to that in CD1d^+/+GalT^−/− mice (n = 4 per group). (C-D) CD1d^−/−CMAH^−/− mice also showed increased anti-NeuGc Ab titer similar to that in CD1d^+/+CMAH^−/− mice (n = 4 per group). Median fluorescence intensity values were used to follow Ab levels. The average values ± SEM for the individual groups are shown.