Figure 7
![Figure 7. Specificity of the Blockmir. (A) Western blot analyses of VE-cadherin, SEMA6A from HUVECs transfected with the LNA27, CD5-2, or control; α-tubulin was used as a loading control. (B) Quantitative analysis as described in (A): control (white bar), LNA27 (hatched bar), and CD5-2 (black bar); mean ± SEM, *P < .05 (n = 3-5). (C) VE-cadherin and (D) PPARγ reporter activity from cells co-transfected with mimic control (Vector[−]) or miR-27a-mimic only (Vector), or miR-27a-mimic plus Blockmirs control or CD5-2. (C) Mean ± SD from 3 independent experiments with 3 replicate samples per experiment; analysis of variance *P ≤ .05. (D) *P < .05 from a representative experiment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/122/16/10.1182_blood-2012-12-473017/4/2911f7.jpeg?Expires=1722691689&Signature=TANQS-ZhBv3hALWxZsduiShOh9JZPzkaxXAXrD44EUnesNW~vkIqjiyPTonCxvJum1pSGb70u6T0wM1PrTgFBFCv0oasW5q4ezT5e1xQnO6gZqLgUSZZOS8bJGh1UUSzQneab-rkMQ61vuOWAAD2CDoKzNS62upL5AjzWXA1i-e~yG0pcEj0XGKSaM2P7MogwYsK0O-SUyDnwUR~ltmuBI2hw1Tep-tQNeLmfY83-nU-x620HmVjWezhexGiJ3oX8cGCyZCgzOPrZBdAr07X2uGZwM9Ty5oxnhinZ1S11gtbYCCyZ6VD4k3MhGe9OAQ9mBtMyEl6jnn8iJnALSQUnw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Specificity of the Blockmir. (A) Western blot analyses of VE-cadherin, SEMA6A from HUVECs transfected with the LNA27, CD5-2, or control; α-tubulin was used as a loading control. (B) Quantitative analysis as described in (A): control (white bar), LNA27 (hatched bar), and CD5-2 (black bar); mean ± SEM, *P < .05 (n = 3-5). (C) VE-cadherin and (D) PPARγ reporter activity from cells co-transfected with mimic control (Vector[−]) or miR-27a-mimic only (Vector), or miR-27a-mimic plus Blockmirs control or CD5-2. (C) Mean ± SD from 3 independent experiments with 3 replicate samples per experiment; analysis of variance *P ≤ .05. (D) *P < .05 from a representative experiment.
