Blockmirs regulate VE-cadherin–dependent functions. (A) HUVECs were stained for VE-cadherin 48 hours after transfection. (i) Control or (ii) CD5-2. White arrows indicate intercellular gaps. (B) Cells were transfected with control or CD5-2, and permeability was assessed through unstimulated or thrombin-stimulated monolayers. Results are normalized to permeability of control (n = 1). The mean ± SEM of 3 to 5 experiments is shown. **P < .005. (C) The Miles assay was performed in mice with control (black bars) or CD5-2 (white bars) injected intravenously. 24 hours later, VEGF or phosphate-buffered saline was given intradermally (n = 4 mice per group). **P < .005.