Regulation of miR-27a in human disease. Human liver tissue from 3 patients with cirrhosis was stained for CD31. (A) Neo-angiogenic vessels (neovessels) in the fibrous septa; (ii) the black circle and venules as indicated with black arrows were captured by LCM. The scale bars indicate (i) 15 µm and (ii) 30 µm. (B) RNA was isolated from ECs in either venules or neovessels (Neo) from three patients (1-3). Expression levels of miR-27a were quantified by qRT-PCR and normalized to miR-520d*. Data represent the mean of quadruplicate qRT-PCR reactions ± SEM. *P < .05 Venules vs Neo.