MiR-27a alters VE-cadherin localization and EC permeability. (A-B) HUVEC stained for VE-cadherin 48 hours after transfection. (Ai) Control mimic or (Aii) miR-27a mimic; (Bi) control LNA or (Bii) LNA-27. The scale bar indicates 100 µm. Arrows indicate intercellular gaps. (C) Permeability measured in control or miR-27a-mimic–transfected cells after 48 hours. Results shown are the normalized means of 5 independent HUVEC lines ± SEM.*P < .05 cf control. (D) 48 hours after transfection with control LNA (black) or miR-27 LNA–transfected cells (white) permeability was measured without (NIL) or after thrombin stimulation (T). Results are from one experiment representative of three performed mean ± SEM *P< 0.05. (E) The Miles assay was performed with 4 µg of control (black) or anti-miR-27a (white bars) injected intradermally into the backs of the mice. 24 hours later, VEGF or phosphate-buffered saline (NIL) was given into the same site. *P < .05, **P < .005, n = 9 mice per group. (F) The levels of miR-27a compared with U6B as assessed in the skin tissue of mice given either control LNA + VEGF (black bar) or LNA-27 + VEGF (white bar). Results are the mean ± SEM of duplicate determinations from 3 mice per treatment. **P < .005