NRF2 and KLF2 bind HS2 of β-globin LCR in K562 cells. (A) NRF2 ChIP analysis of K562 cells treated with 25μM tBHQ for 0, 4, and 6 hours. (B) KLF2 ChIP analysis of K562 cells treated with 5μM statin or 5μM statin + 25μM tBHQ for 24 hours. qPCR was performed on immunoprecipitated DNA using primers that amplify HS5, HS4, HS3, HS2, γ-globin, and the β-globin promoter. The necdin promoter was used as a negative control and the NQO1 promoter was used as a positive control for the NRF2 ChIP. Results are expressed as the fold enrichment over IgG. Error bars represent ± SEM. *P < .05 compared with 0 hours or 0μM (n = 2).