Platelet life span is reduced in VWD type 2B mice with respect to wt-mVWF–expressing mice. Mice expressing wt-mVWF (A-B, closed circles), p.V1316M-mVWF (A, open circles), or p.R1306Q-mVWF (B, open squares) were infused with N-hydroxysuccinimide-biotin, which allows platelet biotinylation. Residual biotinylated platelets were quantified by flow cytometry. Data are expressed as the percentage of biotinylated platelets relative to the total CD41-positive platelet population with t = 0 being arbitrarily set at 100%. Data represent mean ± standard deviation. N = 3 to 13 mice.