Figure 5
Platelets colocalize with macrophages in liver and spleen of VWD type 2B mice. Platelets were isolated from wt-mVWF–expressing or p.V1316M-mVWF–expressing mice, stained with CMTMR cell tracker and reinfused (8 × 107 platelets per mouse) in wt-mVWF–expressing or p.V1316M-mVWF–expressing mice, respectively. Two hours after platelet infusion, liver and spleen were isolated to prepare cryosections. Macrophage subpopulations were identified via immunofluorescence: CD68-positive resident macrophages (Kupffer cells) in liver (A) and CD169-positive marginal metallophilic macrophages in spleen (B). Macrophages are in red, CMTMR-fluorescent platelets are in green, and nuclei are in blue. Original magnification is ×20.
