Bisulfite analysis of proximal Runx1 promoter. (A) Methylation patterns of the Runx1 P2 promoter in cells derived from E14.5 MEFs, E8.5 YS CD41⁺ (E8.5 YS), E14.5 FL Lin-Sca-1⁺CD48⁻CD150⁺ (E14.5 FL), and adult marrow Lin-c-Kit⁺Sca-1⁺CD150⁺CD48⁻ (KLS + SLAM). Sequencing reactions of individual amplicons are represented by each row of circles. Open circles denote unmethylated CpGs, and solid circles represent methylated CpGs. (B) Methylation patterns in mESCs, day 6 EB c-Kit⁺CD41⁺ (D6 EB), and from OP9 cocultures: GFP⁺c-Kit⁺CD45⁺ cells isolated from IRES-GFP control group at day 6 (D6 + 6 control) and HOXB4-IRES-GFP group at day 6 (D6 + 6 HoxB4) and day 11 (D6 + 11 HoxB4).