Runx1 genomic locus and cell populations for bisulfite analysis. (A) Murine Runx1 locus and CpGs analyzed by bisulfite sequencing. For P1 and P2, CpG number represents distance from transcription start site. For +23, CpG number represents distance from the 3′ end of the polymerase chain reaction (PCR) amplicon. (B) Cell populations isolated in vivo and from ESC differentiation strategy for bisulfite analysis.