Kindlin-3–deficient effectors fail to accumulate in inflamed skin but efficiently accumulate in various lymph nodes draining the inflamed skin tissues. (A) FACS of a 1:1 mixture of CFSE-labeled wt T effectors (Q4) mixed with CMTMR-labeled Kindlin-3–deficient T effectors (Q1) prior to intracardiac injection into recipient wt mice. (B) The number of wt and Kindlin-3–deficient T cells (injected at a 1:1 ratio) accumulated in noninflamed or inflamed skin flank 4 hours after intracardiac injection, determined by fluorescence microscopy of fixed skin sections. Skin inflammation was induced by an intradermal injection of CFA. Each dot represents a separate field (0.18 mm² in size) within inflamed or noninflamed flank areas. Results are from multiple fields of 2 mice. ***P < .0001. (C) The partition of CFSE wt or CMTMR Kindlin-3–deficient T cells inside and outside of the skin vessels determined by platelet endothelial CAM-1 immunostaining of skin vessels. Mean values ± SEM of data from the indicated fields shown in B (n = 23). (D) Relative accumulation of wt or Kindlin-3–deficient T cells in inguinal lymph nodes 4 hours after intracardiac injection determined by FACS of harvested suspensions of lymph nodes collected from 2 mice; n.s., not significant. One experiment is representative of 3 experiments. (E) Relative accumulation of sham or PTx pretreated wt or Kindlin-3–deficient T cells determined as in panel D. *P < .01; **P < .002. (F) Relative accumulation of wt or Kindlin-3–deficient T cells in popliteal lymph nodes 4 hours after intracardiac injection. n = 3; n.s., not significant. The values in panels D to F are the mean ± SEM.