Dose-response curves for hVKORC1 variants measured by the cell culture-based assay. (A-F) Secreted FIX activity data for HEK 293T cells coexpressing FIX together with human wild-type or OACR-associated VKORC1 variants in the presence of various warfarin concentrations (0.00-1.0 µM). Data are mean values for n = 6 measurements and error bars show ±SEM. Data points and fitted curves for hVKORC1 wild-type and missense variants are color-coded according to the legend key in each panel.