Expression of activation markers and proliferative capacity of TH cells. (A) Summary of all activation markers showing proportions of CHL-TH cells expressing all activation markers compared with RLN, with those showing statistically significant differences indicated. (B) Representative flow plots and summary comparing CHL-TH and RLN-TH expression levels for selected markers that showed increased median expression in CHL-TH compared with RLN-TH. Similar proportions of CHL-TH and RLN-TH expressed all other markers. (C) Representative photomicrograph (left, ×40 objective, Olympus BX61 microscope) and bar chart (right) summarizing proliferation CHL, RLN, and tonsil-derived SCSs at 30 and 60 days. (D) Representative flow plots showing capacity of proliferative cells to produce TH1 (TNFα and IFNγ, top) and TH2 (IL-4 and IL-13, bottom) cytokines at 60 days (right) compared with only TH1 at baseline (bottom) having been exposed to IL-2 and IL-4 in the culture medium.