PU-H71 induces tumor responses in mice with PEL. (A) In vivo imaging of mice that received an IP injection of BC3/NFκB-luc cells (day 0) and subsequently were untreated (left) or mice that received PU-H71 IP at a dose of 75 mg/kg per day on days 4-13 and 20-29. (B) Kaplan-Meier survival curves for treated and control mice showed prolonged survival in the treatment arm; mice were euthanized when the developed ascites leading to a >10% increase of body weight in a 1-week period; therefore, survival is directly related to tumor growth and independent of possible reporter effects. (C) Established tumors from 3 mice in the vehicle arm were collected 12 hours after administration of 1 75 mg/kg dose of PU-H71 (right lanes); protein extracts were compared with those from tumors in untreated controls (left lanes). Immunoblot analysis shows a decrease in vivo of a classic Hsp90 client.