Quantitation of mini-spectrin dimer crosslinks. The intensity (log scale) of each crosslinked peptide is plotted for each time point as follows: ▪, WT 0°C; ●, WT RT; ▲L260P, 0°C; ▼, L260P RT. (A) Crosslinked peptides consistent with an open dimer conformation. (B) Crosslinked peptides consistent with a closed dimer conformation. (C) Crosslinked peptides unique to L260P and consistent with a closed dimer conformation.