Evaluation of ex vivo modulation temperature with cryopreserved human UCB CD34⁺ cells with dmPGE₂. (A) Genome-wide expression analysis on Affymetrix U133 plus 2.0 GeneChips of human UCB CD34⁺ cells treated with 10 µM of dmPGE₂ for 2 hours at 4°C, 25°C, and 37°C. RMA log₂ normalized expression levels for cells treated with dmPGE₂ (y-axis) in comparison with vehicle-treated cells (x-axis). There were 2, 19, and 192 probe sets (red) with expression levels changing greater (or less) than fourfold due to dmPGE₂ treatment at 4°C (left), 25°C (center), and 37°C (right), respectively. (B) GO enrichment analysis showing biological processes enriched in the upregulated probes induced by treatment with dmPGE₂ at 37°C. Genes upregulated by dmPGE₂ treatment at 37°C in CD34⁺ are listed for each GO category.