β-catenin binding contributes to disease progression in a BMT model for CML. (A) Survival of mice transplanted with p210 BCR/ABL or p210 BCR/ABL(ΔUBD). Kaplan-Meier curves were generated from 3 independent experiments as indicated. Mantel-Cox tests of the 3 survival curves yielded values of P = .0002 (χ2 = 13.5), P = .0002 (χ2 = 13.9), and P = .0046 (χ2 = 8.0), respectively. (B) Blood smears were performed weekly to monitor disease progression. (C) Results of complete blood counts performed at death. Data shown represent an average of 3 mice and are presented with standard deviations. (D) Spleens that were harvested at time of death were weighed. P values were calculated by using a one-way ANOVA followed by Tukey tests. (E) Spleens were harvested at the indicated times, and processed slides were stained with hematoxylin and eosin. Images were visualized by using a Nikon Eclipse 50i microscope (Nikon, Tokyo, Japan) equipped with a 10×/0.25 numerical aperture and were acquired by using a Go-5 camera (QImaging, Surrey, Canada) and QCapture Pro 6.0 software. (F) WCLs were prepared from spleens collected at time of death. Lysates were immunoprecipitated with a BCR/ABL antibody and examined by western blot as indicated. IgG indicates normal rabbit IgG-conjugated agarose; Abl indicates agarose-conjugated anti-abl antibody. **P < .01; ***P < .001 compared with vector; #P < .05 compared with p210 BCR/ABL.
