Impaired adhesion and thrombus formation of Abcg8−/− HS platelets. (A-B) PPACK-anticoagulated whole blood from Abcg8−/− fed a low or high sterol diet was labeled and perfused on mouse VWF immobilized surface at a shear rate of 2000 s−1. Representative images of adherent platelets (A); percent surface coverage for 11 independent experiments (n = 6 for Abcg8−/− LS and n = 5 for Abcg8−/− HS) (B). (C-D) Pooled blood samples of Abcg8−/− LS (n = 4), Abcg8−/− HS (n = 4), and mouse GPIbα-null platelets were perfused over type I collagen for 120 seconds at 2000 s−1. (C) Representative image of adhesion and thrombus formation at 120 seconds (D) quantitates the time-course of surface coverage (%) as they accumulate in the field of view. Platelet counts and hematocrit of these pooled Abcg8−/− LS, Abcg8−/− HS, and control mGPIbα-null samples were 498 × 109/L, 38.2%; 76 × 109/L, 31.3%; and 194 × 109/L, 31.3%, respectively.
