NY1DD sickle mice with implanted DSFCs were infused with LRS, MP4CO, MP4OX, or Hb (8 mL/kg) 24 hours prehypoxia or 30 minutes post-H/R. Livers were removed in all cases after 1 hour of hypoxia and 4 hours of reoxygenation. Thus, livers were removed 29 hours and 3.5 hours postinfusion as indicated. (A) Western blot of liver nuclear NF-ĸB phospho-p65 29 hours postinfusion. (B) Western blot of liver nuclear Nrf2 expression 29 hours postinfusion. (C) Western blot of liver microsomal HO-1 protein expression 3.5 hours and 29 hours postinfusion. (D) Liver microsomal HO activity 3.5 hours and 29 hours postinfusion and after treatment with both MP4CO (29 hours postinfusion) and pretreatment with HO inhibitor SnPP (40 µmols/kg/d × 3 days, IP). N = 4 mice/group. *P < .025. (E) Microvascular stasis after 1 hour of hypoxia and 1 hour of reoxygenation in mice treated with LRS (IP × 3 days) + LRS (IV, 8 mL/kg, 24 hours prehypoxia), LRS (IP × 3 days) + MP4CO (IV, 8 mL/kg, 24 hours prehypoxia), or SnPP (IP, 40 µmols/kg/d × 3 days) + MP4CO (IV, 8 mL/kg, 24 hours prehypoxia). N = 3 mice/group. *P < .025 LRS (IP) + MP4CO (IV) vs LRS (IP) + LRS (IV).