Thin layer chromatography staining of open column fractions. (A) Anisaldehyde semiquantitative chemical staining. (B) Helix pomatia lectin staining (with approximate sugar sizes indicated on the right of the plate). (C) MAb anti-Fs staining. (D) p-Fs crossreactive MAb anti-A 2-22 (clone AY209) staining. (E) Anti-x₂ (clone TH2) staining. (F) p-Fs and Fs crossreactive MAb anti-A(B) (ES-15) staining. In all panels, total glycolipids are indicated as A_pae#1, A_pae#2, whereas open column fractions are identified by their elution solvent (with the fraction used for structural analysis indicated in black). Fs and p-Fs are purified reference glycolipids. Because of minor migration differences between assays, direct comparison of absolute band positions should be viewed with caution.