Analysis of bone marrow progenitors and B-lymphocyte subsets in 4-week-old Suz12^Plt8/+ mice. (A) Gating strategy and (B) number of viable cells for various B-lymphoid populations in the bone marrow of 4-week-old Suz12^+/+ and Suz12^Plt8/+ mice. (C) Gating strategy and (D) number of bone marrow progenitors in 4-week-old Suz12^+/+ and Suz12^Plt8/+ mice. Data represent mean ± standard deviation. A 2-tailed Student t test was used for comparison between the genotypes (*P < .05). Cell surface markers used to define various subsets are as follows: total B, B220⁺CD19⁺; pre-pro B, CD11c⁻NK1.1⁻CD19⁻B220⁺CD43⁺; pre-B-II, B220⁺CD19⁺cKit⁻CD25⁺IgM⁻; pro + pre-B, B220⁺CD19⁺cKit⁻IgM⁻; immature B, B220^lowIgM⁺; recirculating B, B220^highIgM⁺; LSK, lineage⁻Sca1⁺c-Kit⁺; CD150⁺, LSK CD150⁺; HSC, LSK Flt3⁻; MPP, LSK Flt3^int; LMPP, LSK Flt3^high; CLP, lineage⁻ Sca-1⁺c-Kit^intIL-7Rα⁺Flt3⁺; ALP, CLP Ly6D⁻; BLP, CLP Ly6D⁺.