Development of pathologic diseased state in Hdac1^Δ2/Δ2; Hdac2^Δ2/WT and Hdac1&2^Δ2/Δ2 mice. (A) Kaplan-Meier survival curve. WT (n = 13), Hdac1^Δ2/Δ2 (n = 8), Hdac2^Δ2/Δ2 (n = 11), Hdac1^Δ2/WT; Hdac2^Δ2/Δ2 (n = 5), Hdac1^Δ2/Δ2; Hdac2^Δ2/WT (n = 18, median length of survival = 12 weeks) and Hdac1&2^Δ2/Δ2 (n = 20, median length of survival = 15 weeks). (B) Enlarged thymus (white triangles) and spleen (black triangles) representative of Hdac1^Δ2/Δ2; Hdac2^Δ2/WT and Hdac1&2^Δ2/Δ2 diseased mice. A WT LMC is also shown (left panel) for comparison. (C-D) CD4/CD8 and TCRβ/CD5 expression profiles of thymocytes, splenocytes and lymphocytes from diseased and aged matched WT LMCs. (E) Serial thymic sections from WT LMCs and diseased Hdac1^Δ2/Δ2; Hdac2^Δ2/WT mice stained with hematoxylin and eosin (H&E), or Ki67 counterstained with eosin, (i) cortex, (ii) medulla. (F) H&E-stained sections show infiltration of lung, liver and kidney in diseased Hdac1^Δ2/Δ2; Hdac2^Δ2/WT mice (6/14 cases analyzed). (G) Thymocyte clonality analysis: PCR was used to determine Dβ₁-Jβ₁ and Dβ₂-Jβ₂ rearrangement at the TCRβ locus of tail and thy (thymus) gDNA from the genotypes indicated.