Early CD8α⁺ DC expansion after transplantation depends on Irf8 and Nfil3 but not Id2. (A) Analysis of CD11c⁺CD45RA⁻ DCs from spleens of bone marrow chimeric mice in which Id2^gfp/gfp, Id2^fl/flCD11c^CreT/+, Id2^gfp/gfpNfil3^−/−, or Id2^gfp/gfpIrf8^−/−-deficient bone marrow was transplanted into lethally irradiated recipients (Ly5.1⁺) 15 to 20 days earlier. Cells were stained for various surface molecules and analyzed by flow cytometry. Data are representative of 2 to 3 separate experiments with 2 to 4 mice in each experiment with similar results (n = 6-8 mice per group). (B) Analysis of CD8α expression on DCs in the absence of Nfil3 or Id2. DCs were isolated from spleens of intact Id2^gfpNfil3^−/− or Id2CD11c^CreT/+ mice. Data are representative of 2 experiments with similar results (n = 5 mice per genotype). (C) Proportion (top panel) and total number (bottom panel) of splenic CD8α⁺ DCs recovered from spleens of intact WT and KO mice or bone marrow chimeric (KO→Ly5.1) mice 3 weeks after transplantation. Data are pooled from 2 experiments with 5 mice per genotype; NS indicates not statistically significant.