Figure 5
![Figure 5. Mass spectrometry peptide mapping of in vitro glycated human plasminogen. Data are presented as total ion response chromatograms (m/z 100-2000) from 2 to 90 min. (A-B) Plasminogen before and after glycation by glucose, respectively. (C-E) Selected ion response chromatograms: C, m/z = 410.7 [T17, (M+2)/2] of control plasminogen; D, peptide m/z = 1087.5 [T16-FL-T17, (M+2)/2] of glycated plasminogen; and E, m/z = 489.2 [T64, (M+1)] of control plasminogen. Chromatograms of 3 typical independent replicates.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/122/1/10.1182_blood-2013-04-494641/4/134f5.jpeg?Expires=1722784726&Signature=xq83cVU4o55ntKuE062DLS9aFsJwv1h5I35Qz4GWR5-w3AstAQtB1~oZX-zQCrMZExWah-o4Ttkuco-1Ef8eN7x2lReArKURDMELc9lXRZWx62qB-MnfJHq~9xF420ihGJ03QiMnoEFVahPcv~-rIUrs1EZWZluTY~XrJzrwbQuHJ-M2HjVpyofSq3mbVhgI4m82A13YKznoM7Pvt6gXzkG0H1jk2DzgKUMX5XDD9eEr~A8ypDJDUTxZ0atLBR5INC77B6nQ1xtvQkvUiDy8JlHS2boNoybJYZkfl~gRkdqjnu2lttoaW8el94~02pXv5ued8SmM~e-UtrbpiSX1-g__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Mass spectrometry peptide mapping of in vitro glycated human plasminogen. Data are presented as total ion response chromatograms (m/z 100-2000) from 2 to 90 min. (A-B) Plasminogen before and after glycation by glucose, respectively. (C-E) Selected ion response chromatograms: C, m/z = 410.7 [T17, (M+2)/2] of control plasminogen; D, peptide m/z = 1087.5 [T16-FL-T17, (M+2)/2] of glycated plasminogen; and E, m/z = 489.2 [T64, (M+1)] of control plasminogen. Chromatograms of 3 typical independent replicates.
