Effects of glycemia on plasminogen conversion to plasmin using chromogenic substrate assay. Plasminogen conversion to plasmin initiated by tPA (A) or uPA (B) by monitoring plasmin-induced cleavage of chromogenic substrate S2251. Plasma-purified plasminogen was used from a healthy control pool (n = 20) or individual subjects with T1DM pre- and postintervention (n = 10 each). Results are presented as rate of S2251 hydrolysis (au/min). (C) Time course of conversion of plasminogen to plasmin in the presence of fibrin detected by SDS-PAGE and immunoblotting using anti-plasminogen antibody. (D) Densitometry analysis of bands in (C) representing remaining percentage zymogen plasminogen in sample (n = 10). *P < .05. PLG, plasminogen.