Plasma-purified plasminogen integrity and analysis of fibrinolytic activity. (A) SDS-PAGE (left) and western blot using anti-plasminogen antibody (right). Lanes 1 and 2: commercial plasminogen. Lane 3: plasma-purified plasminogen from a healthy control. Lane 4: plasma-purified plasminogen from a subject with T1DM. (B) Turbidimetric analysis of clot lysis using commercial fibrinogen and plasminogen purified from 10 T1DM samples pre- and postimprovement of glycemia (HbA1c 10.7 and 8.2%, respectively). Plasmin(ogen) fibrinolytic activity from pooled healthy control plasma (n = 20) is also shown. PLG, plasminogen.