GR translational isoforms mediated the distinct glucocorticoid responses in immature and mature DCs. Ectopic expression of the GR-A isoform in immature BMDCs enabled glucocorticoid killing. The efficiency of Amaxa transfection of BMDCs was greater than 50%. Positively transfected cells were harvested using the Moflo cell sorter. DEX (100nM) treatment was 24 hours and dead cells were detected using propidium iodide staining (± SD, n = 3; *significantly greater than CON, P < .05; Student t test). Scale bar, 30 μm.