Decreased survival of NKT cells in the absence of DOCK8. (A) Adoptive transfer of mixed WT (CD45.2/GFP⁺) and WT or DOCK8^cpm/cpm (CPM, CD45.2 GFP⁻) splenic NKT cells into WT (CD45.1) recipients. Gating strategy to define donor cells is illustrated showing recipient and control samples (left). Graphs show the proportion of CD45.2 GFP⁺ and GFP⁻ NKT cells at input and after 24 hours and 9 days in the liver and spleen (right). Bars show arithmetic means based on 5 individual animals and error bars show standard error of the mean. Statistical significance was determined by the Mann-Whitney test with Kruskal-Wallis to correct for multiple comparisons; *P < .05; **P < .01. (B) Relative expression of Bcl-2 and CD18 on liver, spleen, and thymic NKT cells in WT (dark line) and DOCK8^cpm/cpm (CPM, filled) mice, representative of 3 independent experiments including 5 animals. Data have been quantified as mean expression of Bcl-2 and CD18 (WT, white bars; CPM, gray bars). Columns shows arithmetic mean, error bars show standard error of the mean, and statistical significance was tested by unpaired t test; *P < .05; **P < .01; ***P < .001.