Mutation pattern in paired (diagnosis [D]/relapse [R]) samples of 53 NPM1^mut patients. Each column represents an individual patient (UPN numbers are indicated in the top row). Colored bars indicate the presence of a mutation, blank bars represent wild-type for the respective gene, and data not available are indicated by a gray bar. Bright and dark green bars represent heterozygous and homozygous FLT3-ITD mutations, respectively. Bars marked by “X” represent different mutation types found at diagnosis and relapse. “Stability” was calculated by the number of mutations that persisted in relapse divided by all mutations present at diagnosis. “Acquired at Relapse” was calculated by the number of cases that acquired a new mutation at relapse and were not detected at diagnosis divided by the number of cases that did not have the same mutation at diagnosis. Mutations in the same gene but of different type (FLT3-ITD, n = 3; NRAS, n = 1) in a diagnosis/relapse pair are considered as loss and new acquisition of a mutation. For MLL-PTD, TP53, and ASXL1, stability could not be determined because no mutations were detected at diagnosis. na, not applicable.