American Society of Hematology

Figure 4

$Figure 4. Genetic interactions between Men1 and Mll1 in B lymphopoiesis. (A) Representative example of fluorescence-activated cell sorting gates used in panels B-D. Percentages shown are relative to the total BM. (B) Comparison of control (Rag1-cre;Men1f/+) to double heterozygote (Rag1-cre;Men1f/+;Mll1f/+) BM B220+/CD43− populations, n = 7-9 animals per genotype, P = .5111. (C) Comparison of control (black), single knockout (light and dark gray), and double knockout (white) BM B220+/CD43− populations, n = 4-15 animals per genotype. (D) A more severe fraction C-C′ block in double knockouts compared with either Men1 or Mll1 knockouts, n = 4-12 animals per genotype. Two-week-old animals were analyzed and ratios were determined as in Figure 3. Statistical significance was determined using the unpaired Student t test; error bars represent 95% confidence intervals; ***P ≤ .001; **P ≤ .01.$

Genetic interactions between Men1 and Mll1 in B lymphopoiesis. (A) Representative example of fluorescence-activated cell sorting gates used in panels B-D. Percentages shown are relative to the total BM. (B) Comparison of control (Rag1-cre;Men1^f/+) to double heterozygote (Rag1-cre;Men1^f/+;Mll1^f/+) BM B220⁺/CD43⁻ populations, n = 7-9 animals per genotype, P = .5111. (C) Comparison of control (black), single knockout (light and dark gray), and double knockout (white) BM B220⁺/CD43⁻ populations, n = 4-15 animals per genotype. (D) A more severe fraction C-C′ block in double knockouts compared with either Men1 or Mll1 knockouts, n = 4-12 animals per genotype. Two-week-old animals were analyzed and ratios were determined as in Figure 3. Statistical significance was determined using the unpaired Student t test; error bars represent 95% confidence intervals; ***P ≤ .001; **P ≤ .01.

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